Department of Surgery Faculty
Paula K. Shireman, MD
Professor and
Vice Dean for Research, Dielmann Chair in Surgery, UT School of Medicine San Antonio
Division: Vascular Surgery
Email: shireman@uthscsa.edu
Board Certifications:
1991 - National Board of Medical Examiners
1998 - American Board of Surgery - General Surgery
2000 - American Board of Surgery - Vascular Surgery
Education:
2004 - MS, Clinical Investigation, University of Texas Health Science Center at San Antonio
1990 - MD, Indiana University School of Medicine, Indianapolis, IN
1986 - BS, Purdue University, West Lafayette, IN
Postgraduate training:
1999 - Fellowship, Peripheral Vascular Surgery, Loyola University Medical Center, Maywood, IL
1998 - Post Doctoral Fellowship, Peripheral Vascular Surgery Research Fellow, Loyola University
Medical Center, Maywood, IL
1997 - Residency, General Surgery, Northwestern University Medical Center, Chicago, IL
1995 - Post Doctoral Fellowship, Vascular Surgery Research Fellow, Northwestern University
Chicago, IL
Research interests: Our lab studies the inflammatory-mediated mechanisms of angiogenesis and skeletal muscle regeneration. We are particularly interested in how hematopoietic stem cells, myogenic stem cells and inflammatory cells interact to form muscle after injury and how microRNAs regulate this process. A better understanding of muscle regeneration will be useful in tissue regeneration strategies for limb salvage.
Active grant support:
R01 HL074236 09/01/03 to 06/30/2012, NIH/NHLBI
Role: PI
Chemokines and Immune Cells in Hind Limb Ischemia
The major goals of this application are to 1) identify the mechanisms
underlying the differential susceptibility to tissue necrosis in two
inbred strains of mice after femoral artery excision, 2) determine the
role of T-cells and the Th1/Th2 immune response in tissue necrosis and
3) determine the role of the MCP-1/CCR2 axis in tissue necrosis. A
Minority Supplement was also awarded to Oscar Ochoa, M.D. in association
with this grant (09/1/05-08/31/07)
Merit Review 1I01BX001186 10/01/011 to 9/30/15 Veterans Administration
Role: PI
MicroRNA Regulation of Macrophage Polarization in Muscle Regeneration
The major goals of this application are to determine the functions of miR-147
and miR-21 in macrophage function and muscle regeneration.
Completed grant support:
3R01 HL074236-06S1 7/1/09 - 6/30/11
Chemokines and immune cells in hind limb ischemia - ARRA Diversity Supplement to support
the postdoctoral research training of Jason Wells, MD.
VA Merit Review 10-1-2007 to 9-20-2011, Veterans Administration
Role: PI
Macrophage Regulated Muscle Regeneration: Fat Deposition and Function
VA Merit Review 01/01/04 to 9/30/07, Veterans Administration
Role: PI
Role of the MCP-1/CCR2 Axis in Hind Limb Ischemia
The major goals of this application are to determine the mechanisms of
the delay in skeletal muscle regeneration previously observed in MCP-1
-/- mice. Radiation chimeras will be used to determine the influence of
MCP-1 and CCR2 expression in hematopoietic and non-hematopoietic cells.
Select Publications:
McHale MJ, Sarwar ZU, Cardenas DP, Porter L, Salinas AS, Michalek JE, McManus LM,
Shireman PK.
Increased fat deposition in injured skeletal muscle is regulated by sex-specific
hormones.
Am J Physiol Regul Integr Comp Physiol, 302(3):R331-9, 2012. PMID:22116509,
PMCID: PMC 3289262
Chen Y, Gelfond J, McManus LM, Shireman PK. Temporal MicroRNA Expression during in vitro Myogenic Progenitor Cell Proliferation and Differentiation: Regulation of Proliferation by miR-682. Physiol Genomics. 2010 Sep 14. [Epub ahead of print] PMID:20841498, PMCID: PMC3110887
Martinez R, Fierro CA, Shireman PK, Han HC. Mechanical buckling of veins under internal pressure. Ann Biomed Eng, 38(4):1345-53, 2010. PMID 20094913, PMC:2896067.
Prajapati SI, Martinez CO, Abraham J, McCleish AT, Michalek JE, McManus LM, Rubin BP, Shireman PK, Keller C. Crimson carrier, a long-acting contrast agent for in vivo near-infrared imaging of injured and diseased muscle. Muscle & Nerve, 2010, 42(2):245-51. PMID 20544935, PMCID: PMC2910161.
Chen Y, Gelfond JA, McManus LM, Shireman PK. Reproducibility of quantitative RT-PCR array in miRNA expression profiling and comparison with microarray analysis. BMC Genomics, 10(1):407, 2009. PMID: 19715577, PMCID: PMC2753550.
Martinez CO, McHale MJ, Wells JT, Ochoa O, Michalek J, McManus LM, Shireman PK. Regulation of skeletal muscle regeneration by CCR2-activating chemokines is directly related to macrophage recruitment. Am J Physiol Regul Integr Comp Physiol, 2010, 299(3):R832-42. PMID 20631294, PMCID: PMC2630778
Prajapati JI, Martinez CO, Bahadur AN, Wu IQ, Zheng W, Lechleiter JD, McManus LM, Chisolm GB, Michalek JE, Shireman PK, Keller C. A novel near-infrared contrast agent for injured tissues in living subjects. [Cover Article] Molecular Imaging, 8(1): 45-54, 2009. PMID 19344575, PMCID: PMC2790532.
Sun D, Martinez CO, Ochoa O, Ruiz-Willhite L, Bonilla JR, Centonze VE, Waite LL, Michalek JE, McManus LM, Shireman PK. Bone marrow-derived cell regulation of skeletal muscle regeneration. FASEB J, 23(2): 382-95, 2009. PMID 18827026, PMCID: PMC2630778.
Pierce AP, de Waal E, McManus LM, Shireman PK, Chaudhuri AR. Oxidation and structural perturbation of redox-sensitive enzymes in injured skeletal muscle. Free Radic Biol Med. 41(12):1584-93, 2007. PMID: 18037124
Ochoa O, Torres FM, Shireman PK. Chemokines and diabetic wound healing. J Vasc Surg, 2007; 15(6):350-355.
Ochoa O, Sun D, Reyes-Reyna SM, Waite LL, Michalek JE, McManus LM, Shireman PK. Delayed angiogenesis and VEGF production in CCR2 -/- mice during impaired skeletal muscle regeneration. Am J Physiol Reg Int Comp Physiol, 293(2):R651-61, 2007. PMID: 17522124.
Shireman PK. The chemokine system in arteriogenesis and hind limb ischemia. J Vasc Surg, 45(Supplement A):48A-56A, 2007.
Shireman PK, Contreras-Shannon V, Ochoa O, Karia BP, Michalek JE, McManus LM. MCP-1 deficiency causes altered inflammation with impaired skeletal muscle regeneration. J Leuk Biol, 81:775-85, 2007.
Contreras-Shannon V, Ochoa O, Reyes-Reyna SM, Sun D, Michalek JE, Kuziel WA, McManus LM, Shireman PK. Fat accumulation with altered inflammation and regeneration in skeletal muscle of CCR2 -/- mice following ischemic injury. Am J Physiol Cell Physiol, 292:C953-67, 2007.
Lee S, Shin S, Shireman PK, Vasilaki A, Van Remmen H, Csete M. Glutathione-peroxidase-1 null muscle progenitor cells are globally defective. Free Radic Biol Med. 41:1174-84, 2006.
LaFontaine J, Allen M, Davis C, Harkless LB, Shireman PK. Current concepts in diabetic microvascular dysfunction. J Am Podiatr Med Assoc. 96(3):245-52, 2006.
Shireman PK, Contreras-Shannon VE, Reyes-Reyna SM, Robinson SC, McManus LM. MCP-1 parallels inflammatory and regenerative responses in ischemic muscle. J Surg Res. 134(1):145-57, 2006.
Shireman PK, Quinones MP. Differential necrosis despite similar perfusion in mouse strains after ischemia, J Surg Res, 2005 Dec
Toursarkissian B, Hagino RT, Khan K, Schoolfield J, Shireman PK, Harkless L. Healing of transmetatarsal amputation in the diabetic patient: is angiography predictive? Ann Vasc Surg 2005 Nov:19(6):769-73.
Presentations:
Chen Y, Gelfond JA, McManus LM, Shireman PK. MiRNA expression patterns during skeletal
muscle regeneration San Antonio, TX: 2010 May. (Annual Terry M. Mikiten Graduate Student
Research).
Chen Y, Gelfond JA, McManus LM, Shireman PK. MiRNA expression patterns during skeletal muscle regeneration. Anaheim, CA 2010 Apr. (Experimental Biology).
Chen Y, Gelfond JA, McManus LM, Shireman PK. MiRNA expression patterns during skeletal muscle regeneration Anaheim, CA: 2010 Apr. (Experimental Biology).
McHale MJ, Schlechter AK, Torres FM, Michalek JE, McManus LM, Shireman PK. Gender-specific differences in skeletal muscle regeneration, presented at the Academic Surgical Congress, Association for Academic Surgery, 2010 Feb. p. 218. (J Surg Res; vol. 158, no. 2).
McHale M, Schlecter AK, Torres FM, Michalek JE, McManus LM, Shireman PK. Increased fat accumulation in regenerating skeletal muscle in females 2009 Aug. (Annual Meeting of the San Antonio Vascular Surgery Society).
Martinez CO, McManus LM, Shireman PK. CCR2 influence in skeletal muscle recovery from injury 2009 Jun. (Aust Surgical Society).
Chen Y, Gelfond JA, McManus LM, Shireman PK. Differential microRNA expression during myogenic progenitor cell proliferation and differentiation. Proceedings of the Joint Meeting 2009 May. (Frontiers in Myogenesis and Skeletal Muscle Satellite and Stem Cells, New York, NY).
Martinez CO, McHale MJ, Ochoa O, McManus LM, Shireman PK. Early macrophage recruitment is directly related to regenerated skeletal muscle fiber size 2009 May. (Making Muscle in the Embryo and Adult).
Chen Y, Gelfond JA, McManus LM, Shireman PK. Differential microRNA expression during myogenic progenitor cell proliferation and differentiation New York, NY: 2009 May. (Meeting of Frontiers in Myogenesis and Skeletal Muscle Satellite and Stem Cells).
Martinez CO, McManus LM, Shireman PK. Kinetics of myogenic progenitor cell (MPC) expansion and bone marrow-derived cell recruitment in skeletal muscle regeneration 2009 Apr. (Experimental Biology).
Chen Y, Gelfond JA, McManus LM, Shireman PK. Reproducibility of miRNA expression by proliferating C2C12 cells using RT-PCR array 2009 Apr. (Platform Presentation at Experimental Biology).
Chen Y, Gelfond JA, McManus LM, Shireman PK. Reproducibility of miRNA expression by proliferating C2C12 cells using RT-PCR Array New Orleans, LA: 2009 Apr. (2009 Experimental Biology).
Chen Y, Gelfond JA, McManus LM, Shireman PK. MicroRNA profiling in murine myoblasts using quantitative RT-PCR array. 2009 Mar. (UTHSCSA Graduate Student Association sponsored Research Day).
Martinez CO, McManus LM, Shireman PK. Myogenic progenitor cell and bone marrow-derived cell kinetics in skeletal muscle regeneration 2009 Mar. (Proceedings of the American College of Surgery).
Chen Y, Gelfond JA, McManus LM, Shireman PK. microRNA profiling in murine myoblasts using quantitative RE-PCR array San Antonio, TX: 2009 Mar. (Annual Terry M. Mikiten, PHD Graduate Student Research Forum).
McHale M, Schlecter AK, Torres FM, Michalek JE, McManus LM, Shireman PK. Gender-Dependent Fat Accumulation in Regenerating Skeletal Muscle. 2009 Jan. (Aust Surgical Society).
Martinez CO, Sun D, Waite LL, Michalek JE, McManus LM, Shireman PK. Differential regulation of skeletal muscle regeneration by CCR2-activating chemokines and in vivo characteristics of myogenic progenitor cells 2008 Oct. (Annual Meeting of the San Antonio Vascular Surgery Society).
Schlechter AK, Torres FM, McManus LM, Shireman PK. Altered muscle regeneration in the absence of CC chemokine receptor 2. 2008 Sep. (8th Annual Medical Student Research Day, UTHSCSA).
Ochoa O, Martinez C, Torres FM, Ruiz-Willhite L, Michalek JE, McManus LM, Shireman PK. Differential regulation of skeletal muscle regeneration and angiogenesis by CCR2-activating chemokines, presented at the Academic Surgical Congress, Association for Academic Surgery, 2008 Feb. p. 218-219. (J Surg Res; vol. 144, no. 2).
Sun D, Wetzel MD, Waite LL, Michalek JE, Shireman PK. Environmental factors determine in vivo myogenic progenitor cell function, presented at the Academic Surgical Congress, Society of University Surgeons, 2008 Feb. p. 217-218. (J Surg Res; vol. 144, no. 2).
Shireman PK, Sun D, Ochoa O, Bonilla JR, Waite LL, Michalek JE, McManus LM. Increased myogenic progenitor cell accumulation in CCR2 -/- mice despite bone marrow-dependent impairments in muscle regeneration and macrophage recruitment 2007 Jul. (Skeletal Muscle Satellite & Stem Cells, FASEB Summer Meeting).
Sun D, Ochoa O, McManus LM, Shireman PK. Bone marrow (BM) transplantation corrects impaired macrophage recruitment and muscle regeneration in CC chemokine receptor 2 (CCR2) -/- mice, presented at the Academic Surgical Congress, Association for Academic Surgery, Plenary Session, 2007 Feb. p. 180-181. (J Surg Res; vol. 137, no. 2).
Ochoa O, Solomon D, Michalek JE, McManus LM, Shireman PK. Similar capillary density despite decreased vascular endothelial growth factor (VEGF) levels and impaired skeletal muscle regeneration in CC chemokine receptor 2 (CCR2) -/- mice, presented at the Academic Surgical Congress, Society of University Surgeons 2007 Feb. p. 211-212. (J Surg Res; vol. 137, no. 2).
Shireman PK, Sun D, Ochoa O, Bonilla JR, Waite LL, Michalek JE, McManus LM. Bone marrow- derived cell regulation of skeletal muscle regeneration. 2007. (Proceedings of the International Conference on Age-related Muscle Atrophy: Causes and Mechanisms, Bandera, TX).
FASEB 2007 - Skeletal Muscle Satellite & Stem Cells, July 2007. "Increased myogenic progenitor cell accumulation in CCR2-/- mice despite bone marrow-dependent impairment in muscle regeneration and macrophage recruitment."
Contreras-Shannon, V, McManus LM, Shireman PK. Ischemia-induced enhanced transcription factor (myoD and STAT3) expression in MCP-1 -/- mice despite decreased skeletal muscle regeneration, to be presented at the Society of University Surgeons Meeting, San Diego, CA, 2006 Feb.
Pierce A, McManus LM, Shireman PK, Chaudhuri AR. MPO activity is inversely related to protein structure and function in injured and regenerating muscle, presented at The Role of Protein Misfolding and Aggregation on Aging and Age-Related Disorders, Bandera, TX, 2005 Oct.
Shireman PK, Contreras-Shannon V, McManus LM. Macrophages and muscle regeneration after ischemic injury, presented at the North American Vascular Biology Organization Meeting, Chicago, IL, 2005 Jun. p. 163. (Vascular Medicine; vol. 10, no. 2).
Contreras-Shannon V, McManus LM, Kuziel WA, Shireman PK. Alterations in STAT3 expression during skeletal muscle regeneration in CC chemokine receptor 2 knockout (CCR2 -/-) mice, presented at the FASEB Skeletal Muscle Satellite and Stem Cell Conference, Tucson, AZ, 2005 Jun.
Contreras-Shannon V, McManus LM, Kuziel WA, Shireman PK. Alterations in MyoD expression during skeletal muscle regeneration following cardiotoxin (CT) induced injury in CC chemokine receptor 2 (CCR2 -/-) mice, presented at the Experimental Biology Meeting, San Diego, CA, 2005 Apr. p. A486. (FASEB J; vol. 19, no. 4).
Biosketch: Dr. Shireman obtained her medical degree from Indiana University School of Medicine in Indianapolis, IN in 1990 and completed her Vascular Surgery training at Loyola University Medical Center in Maywood, IL in 1998. She is currently certified with the American Board of Surgery in both General Surgery and Vascular Surgery. Dr. Shireman joined the Vascular Surgery Division at the Health Science Center in 1999. After arriving at the Health Science Center, Dr. Shireman completed her masters program in Clinical Investigation and received numerous grants for research from the NIH, the Department of Veteran Affairs, Howard Hughes Medical Institute, the American Heart Association, and the University of Texas Health Science Center at San Antonio. In 2004 Dr. Shireman was appointed to the faculty for the Barshop Institute for Longevity and Aging Studies. She currently is serving as the Vice Dean for Research for the School of Medicine where she is a tenured Professor in the Department of Surgery. Dr. Shireman performs open and endovascular procedures as well as trains residents and medical students. Her published research is primarily in macrophage biology, angiogenesis, stem cells, tissue regeneration and microRNAs. In her role role as Vice Dean, she is responsible for 5 research centers/institutes, facilitating a telemedicine initiative, integrating research and educational activities along the South Texas Border in conjunction with the Regional Academic Health Center in Edinburg and Harlingen, as well as promoting research activities for the School of Medicine.
Honors and awards:
2012, American Heart Association National Research Committee
2012, Dielmann Chair in Surgery
2009, American Heart Association South Central Affiliate - Chairman, Research Committee and
board Member
2004, Outstanding Alumni Award, Purdue University, West Lafayette, IN
2004, Presidential Junior Research Scholar Award, University of Texas Health Science Center
at San Antonio
2001, Alumni Hidden Diamond Award, Purdue University, West Lafayette, IN
1999, John L. Keeley Surgical Fellowship Award, Loyola University Medical School, Maywood, IL
1998, Gutherie Award for Research, Midwestern Vascular Surgical Society, Dearborn, MI
1997, Research Award, 3rd Place, American Venous Forum, San Antonio, TX
1995, Residents' Research Award, The Chicago Surgical Society, Chicago, IL
Committees, Professional Societies:
Association of Women Surgeons, member
Association for Academic Surgery, member
American College of Surgeons, fellow
The Association of Veterans Administrative Surgeons, member
North American Vascular Biology Organization (NAVBO), member
Society for Vascular Surgery, distinguished fellow
4B Study Section Member, American Heart Association, Western Consortium
Research and Education Committee, American Vascular Association, member
American Society for Investigative Pathology
Research Allocations & Advisory Committee, American Heart Assoc., TX Affiliate, member
Society of University Surgeons
