Department of Surgery Faculty
Paula K. Shireman, MD
Vice Dean for Research, Dielmann Chair in Surgery, UT School of Medicine San Antonio
Division: Vascular and Endovascular Surgery
1991 - National Board of Medical Examiners
1998 - American Board of Surgery - General Surgery
2000 - American Board of Surgery - Vascular Surgery
2004 - MS, Clinical Investigation, University of Texas Health Science Center at San Antonio
1990 - MD, Indiana University School of Medicine, Indianapolis, IN
1986 - BS, Purdue University, West Lafayette, IN
1998-1999 - Fellowship, Peripheral Vascular Surgery, Loyola University Medical Center, Maywood, IL
1997-1998 - Post Doctoral Fellowship, Peripheral Vascular Surgery Research Fellow, Loyola University Medical Center, Maywood, IL
1993-1995 - Post Doctoral Fellowship, Vascular Surgery Research Fellow, Northwestern University Chicago, IL
1990-1997 - Residency, General Surgery, Northwestern University Medical Center, Chicago, IL
Research interests: Our lab studies the inflammatory-mediated mechanisms of angiogenesis and skeletal muscle regeneration. We are particularly interested in how hematopoietic stem cells, myogenic stem cells and inflammatory cells interact to form muscle after injury and how microRNAs regulate this process. A better understanding of muscle regeneration will be useful in tissue regeneration strategies for limb salvage.
Biosketch: I am an academic vascular surgeon and basic/translational science researcher, currently serving as the Vice Dean for Research for the School of Medicine at the University of Texas Health Science Center at San Antonio. I hold the Dielmann Chair in Surgery endowment and am a tenured Professor in the Department of Surgery. In my clinical role, I perform open and endovascular procedures while training residents and medical students.
As a researcher, I am the principal investigator for grants from the National Institutes of Health and the Veterans Administration. My published research is primarily in macrophage biology, angiogenesis, stem cells, tissue regeneration and microRNAs. Our translational research incorporates medical, graduate and postdoctoral student training. I collaborate with investigators at the San Antonio Military Health System to determine the long- term outcomes of Service Members with extremity vascular injuries to improve battlefield treatments and transitions of care between the Department of Defense and Veterans Administration.
I am a co-Principal Investigator on our NIH Clinical Translational Science Award responsible for strategic partnership with our CTSA affiliated institutions, biostatistics, study design and biomedical informatics cores, and evaluation of all CTSA programs.
In my role as Vice Dean, I am responsible for 5 research centers/institutes, research faculty recruitments/retentions, integrating research and educational activities along the South Texas Border in conjunction with the Regional Academic Health Center in Harlingen and Edinburg during their transition to becoming an independent medical school, as well as promoting research activities for the School of Medicine and our partner institutions.
Funding Agency: Veterans Administration
Title: VA Vascular Injury Study (VAVIS): VA-DOD extremity injury outcomes collaboration
Grant number: 1IO1HX001304
Period: 01/2014 - 12/2016
Role: Co-Principal Investigator
Grant Detail: This grant will define long-term outcomes of extremity vascular repairs which are currently not available in the civilian or military trauma literature in regards to limb function and repair patency. Furthermore, monitoring of the repair with duplex ultrasound and drug therapies such as antiplatelet agents to improve patency rates are based upon studies in patients with atherosclerosis as the etiology of the vascular repair rather than traumatic injury. To better understand the outcomes and needs of this group of Veterans, the following 3 aims will be performed: 1) Compare injury, demographic and geospatial characteristics of patients with initial in-theatre limb salvage (IILS) and identify late vascular surgery related limb complications and health care utilization in Veterans receiving VA vs. non-VA care. 2) Characterize the preventive services received by individuals with vascular repair and related outcomes. 3) Describe patient-reported functional outcomes in Veterans with traumatic vascular limb injuries. This application received an excellent impact score (149). We have been notified of the intent to fund the application after Just in Time information has been approved.
Funding agency: NIH/NHLBI
Title: Improved Muscle Regeneration by Anti-miRNA Alteration in Macrophage Polarization
Grant number: F30 HL220743
Period: 7/2011 - 6/2016
Grant detail: MD/PhD training grant to support the training of David Melton in macrophage biology and microRNA functions, Dr. Shireman serves as the mentor for this award.
Funding agency: NIH/NCATS
Title: Institute for Integration of Medicine & Science: A Partnership to Improve Health
Grant number: UL1 TR000149=05
Period: 09/2013 - 04/2018
Role: Co-Principal Investigator
Grant detail: This Clinical and Translational Science Award provides a broad range of research infrastructure support. Dr. Shireman is responsible for programs in biostatistics, bioinformatics, evaluation for the CTSA as well as managing relationships with partnering institutions
Funding agency: NIH / NHLBI
Title: Pathobiology of Occlusive Vascular Disease
Grant number: T32 HL07446
Period: 9/2012 - 8/2017
Grant detail: This NIH training grant supports postdoctoral training in the area of occlusive cardiovascular disease, inflammation, and degenerative disorders of cardiovascular tissues.
Funding agency: NIH/NIGMS
Title: Injury, ischemia and inflammation: A translation approach to trauma treatment
Grant number: T32 GM079085
Period: 7/2008 - 6/2013
Grant detail: Department of Surgery institutional T32 training grant. The Principal Investigator is Martin Schwacha, PhD; Dr. Shireman is a preceptor for this grant.
Funding agency: Veterans Administration
Title: MicroRNA Regulation of Macrophage Polarization in Muscle Regeneration
Grant number: Merit Review 1I01BX001186
Period: 10/01/011 to 9/30/15
Grant detail: The major goals of this application are to determine the functions of miR-147 and miR-21 in macrophage function and muscle regeneration.
Select completed grant support:
Funding agency: NIH/NHLBI
Title: Chemokines and Immune Cells in Hind Limb Ischemia
Grant number: R01 HL074236
Period: 09/01/03 to 06/30/2013
Grant detail: The major goals of this application are to 1) identify the mechanisms underlying the differential susceptibility to tissue necrosis in two inbred strains of mice after femoral artery excision, 2) determine the role of T-cells and the Th1/Th2 immune response in tissue necrosis and 3) determine the role of the MCP-1/CCR2 axis in tissue necrosis. A Diversity Supplement was also awarded to Oscar Ochoa, M.D. in association with this grant (09/1/05-08/31/07) and Jason Wells, MD (07/01/09-06/30/11).
Funding agency: NIH/NIAMS
Title: Regulation of muscle regeneration by microRNAs
Grant number: K01 AR059096
Period: 9/2010 - 6/2011
Grant detail: This career development award supports Yongxin Chen, MD, PhD as he establishes a research program studying microRNA regulation of myogenic progenitor cell proliferation and differentiation. Dr. Shireman served as the mentor for Dr. Chen. The grant was ended by Dr. Chen to pursue a Pathology Residency.
Period: 7/1/09 - 6/30/11
Chemokines and immune cells in hind limb ischemia - ARRA Diversity Supplement to support the postdoctoral research training of Jason Wells, MD.
VA Merit Review, Veterans Administration
Period: 10-1-2007 to 9-20-2011
Macrophage Regulated Muscle Regeneration: Fat Deposition and Function
VA Merit Review, Veterans Administration
Period: 01/01/04 to 9/30/07
Role of the MCP-1/CCR2 Axis in Hind Limb Ischemia
The major goals of this application are to determine the mechanisms of the delay in skeletal muscle regeneration previously observed in MCP-1 -/- mice. Radiation chimeras will be used to determine the influence of MCP-1 and CCR2 expression in hematopoietic and non-hematopoietic cells.
Chen Y, Melton DW, Gelfond JA, McManus LM, Shireman PK. MiR-351 transiently increases during muscle regeneration and promotes progenitor cell proliferation and survival upon differentiation. Physiol Genomics. 2012 Nov 1;44(21):1042-51. doi: 10.1152/physiolgenomics.00052.2012. Epub 2012 Sep 11.
McHale MJ, Sarwar ZU, Cardenas DP, Porter L, Salinas AS, Michalek JE, McManus LM, Shireman PK. Increased fat deposition in injured skeletal muscle is regulated by sex-specific hormones. Am J Physiol Regul Integr Comp Physiol, 302(3):R331-9, 2012. PMID:22116509, PMCID: PMC 3289262
Chen Y, Gelfond J, McManus LM, Shireman PK. Temporal MicroRNA Expression during in vitro Myogenic Progenitor Cell Proliferation and Differentiation: Regulation of Proliferation by miR-682. Physiol Genomics. 2010 Sep 14. [Epub ahead of print] PMID:20841498, PMCID: PMC3110887
Martinez R, Fierro CA, Shireman PK, Han HC. Mechanical buckling of veins under internal pressure. Ann Biomed Eng, 38(4):1345-53, 2010. PMID 20094913, PMC:2896067.
Prajapati SI, Martinez CO, Abraham J, McCleish AT, Michalek JE, McManus LM, Rubin BP, Shireman PK, Keller C. Crimson carrier, a long-acting contrast agent for in vivo near-infrared imaging of injured and diseased muscle. Muscle & Nerve, 2010, 42(2):245-51. PMID 20544935, PMCID: PMC2910161.
Chen Y, Gelfond JA, McManus LM, Shireman PK. Reproducibility of quantitative RT-PCR array in miRNA expression profiling and comparison with microarray analysis. BMC Genomics, 10(1):407, 2009. PMID: 19715577, PMCID: PMC2753550.
Martinez CO, McHale MJ, Wells JT, Ochoa O, Michalek J, McManus LM, Shireman PK. Regulation of skeletal muscle regeneration by CCR2-activating chemokines is directly related to macrophage recruitment. Am J Physiol Regul Integr Comp Physiol, 2010, 299(3):R832-42. PMID 20631294, PMCID: PMC2630778
Prajapati JI, Martinez CO, Bahadur AN, Wu IQ, Zheng W, Lechleiter JD, McManus LM, Chisolm GB, Michalek JE, Shireman PK, Keller C. A novel near-infrared contrast agent for injured tissues in living subjects. [Cover Article] Molecular Imaging, 8(1): 45-54, 2009. PMID 19344575, PMCID: PMC2790532.
Sun D, Martinez CO, Ochoa O, Ruiz-Willhite L, Bonilla JR, Centonze VE, Waite LL, Michalek JE, McManus LM, Shireman PK. Bone marrow-derived cell regulation of skeletal muscle regeneration. FASEB J, 23(2): 382-95, 2009. PMID 18827026, PMCID: PMC2630778.
Pierce AP, de Waal E, McManus LM, Shireman PK, Chaudhuri AR. Oxidation and structural perturbation of redox-sensitive enzymes in injured skeletal muscle. Free Radic Biol Med. 41(12):1584-93, 2007. PMID: 18037124
Ochoa O, Torres FM, Shireman PK. Chemokines and diabetic wound healing. J Vasc Surg, 2007; 15(6):350-355.
Ochoa O, Sun D, Reyes-Reyna SM, Waite LL, Michalek JE, McManus LM, Shireman PK. Delayed angiogenesis and VEGF production in CCR2 -/- mice during impaired skeletal muscle regeneration. Am J Physiol Reg Int Comp Physiol, 293(2):R651-61, 2007. PMID: 17522124.
Shireman PK. The chemokine system in arteriogenesis and hind limb ischemia. J Vasc Surg, 45(Supplement A):48A-56A, 2007.
Shireman PK, Contreras-Shannon V, Ochoa O, Karia BP, Michalek JE, McManus LM. MCP-1 deficiency causes altered inflammation with impaired skeletal muscle regeneration. J Leuk Biol, 81:775-85, 2007.
Contreras-Shannon V, Ochoa O, Reyes-Reyna SM, Sun D, Michalek JE, Kuziel WA, McManus LM, Shireman PK. Fat accumulation with altered inflammation and regeneration in skeletal muscle of CCR2 -/- mice following ischemic injury. Am J Physiol Cell Physiol, 292:C953-67, 2007.
Lee S, Shin S, Shireman PK, Vasilaki A, Van Remmen H, Csete M. Glutathione-peroxidase-1 null muscle progenitor cells are globally defective. Free Radic Biol Med. 41:1174-84, 2006.
LaFontaine J, Allen M, Davis C, Harkless LB, Shireman PK. Current concepts in diabetic microvascular dysfunction. J Am Podiatr Med Assoc. 96(3):245-52, 2006.
Shireman PK, Contreras-Shannon VE, Reyes-Reyna SM, Robinson SC, McManus LM. MCP-1 parallels inflammatory and regenerative responses in ischemic muscle. J Surg Res. 134(1):145-57, 2006.
Shireman PK, Quinones MP. Differential necrosis despite similar perfusion in mouse strains after ischemia, J Surg Res, 2005 Dec
Toursarkissian B, Hagino RT, Khan K, Schoolfield J, Shireman PK, Harkless L. Healing of transmetatarsal amputation in the diabetic patient: is angiography predictive? Ann Vasc Surg 2005 Nov:19(6):769-73.
Honors and awards:
2012, Dielmann Chair in Surgery (endowment)
2009, American Heart Association South Central Affiliate - Chairman, Research Committee and board Member
2004, Outstanding Alumni Award, Purdue University, West Lafayette, IN
2004, Presidential Junior Research Scholar Award, University of Texas Health Science Center at San Antonio
2001, Alumni Hidden Diamond Award, Purdue University, West Lafayette, IN
1999, John L. Keeley Surgical Fellowship Award, Loyola University Medical School, Maywood, IL
1998, Gutherie Award for Research, Midwestern Vascular Surgical Society, Dearborn, MI
1997, Research Award, 3rd Place, American Venous Forum, San Antonio, TX
1995, Residents' Research Award, The Chicago Surgical Society, Chicago, IL
Committees, Professional Societies:
American Heart Association National Research Committee, member
American Surgical Association, member
American Heart Association, Fellow
Association of Women Surgeons, member
Association for Academic Surgery, member
American College of Surgeons, fellow
The Association of Veterans Administrative Surgeons, member
North American Vascular Biology Organization (NAVBO), member
Society for Vascular Surgery, distinguished fellow
4B Study Section Member, American Heart Association, Western Consortium
Research and Education Committee, American Vascular Association, member
American Society for Investigative Pathology
Society of University Surgeons
Bioengineering, Technology and Surgical Sciences (BTSS) NIH Study Section, member