Department of Surgery Faculty

Paula K. Shireman, MD

Associate Professor

Division: Vascular Surgery
Email: shireman@uthscsa.edu

Board Certifications:
1991 - National Board of Medical Examiners
1998 - American Board of Surgery - General Surgery
2000 - American Board of Surgery - Vascular Surgery

Education:
2004 - MS, Clinical Investigation, University of Texas Health Science Center at San Antonio
1990 - MD, Indiana University School of Medicine, Indianapolis, IN
1986 - BS, Purdue University, West Lafayette, IN

Postgraduate training:
1999 - Post Doctoral Fellowship, Vascular Surgery, Loyola University Medical Center, Maywood, IL
1998 - Post Doctoral Fellowship, Peripheral Vascular Surgery Research Fellow, Loyola University Medical Center, Maywood, IL
1997 - Residency, General Surgery, Northwestern University Medical Center, Chicago, IL
1995 - Post Doctoral Fellowship, Vascular Surgery Research Fellow, Northwestern University Chicago, IL

Research interests: Our lab studies the inflammatory-mediated mechanisms of angiogenesis and skeletal muscle regeneration. We are particularly interested in how hematopoietic stem cells, myogenic stem cells and inflammatory cells interact to form muscle after injury. A better understanding of muscle regeneration will be useful in tissue regeneration strategies for limb salvage.

Active grant support:

VA Merit Review 10-1-2007 to 9-20-2011, Veterans Administration
Role: PI
Macrophage Regulated Muscle Regeneration: Fat Deposition and Function

R01 HL074236 09/01/03 to 08/31/07, NIH/NHLBI
Role: PI
Chemokines and Immune Cells in Hind Limb Ischemia
The major goals of this application are to 1) identify the mechanisms underlying the differential susceptibility to tissue necrosis in two inbred strains of mice after femoral artery excision, 2) determine the role of T-cells and the Th1/Th2 immune response in tissue necrosis and 3) determine the role of the MCP-1/CCR2 axis in tissue necrosis. A Minority Supplement was also awarded to Oscar Ochoa, M.D. in association with this grant (09/1/05-08/31/07)

VA Merit Review 01/01/04 to 9/30/07, Veterans Administration
Role: PI
Role of the MCP-1/CCR2 Axis in Hind Limb Ischemia
The major goals of this application are to determine the mechanisms of the delay in skeletal muscle regeneration previously observed in MCP-1 -/- mice. Radiation chimeras will be used to determine the influence of MCP-1 and CCR2 expression in hematopoietic and non-hematopoietic cells.

Presentations:
FASEB 2007 - Skeletal Muscle Satellite & Stem Cells, July 2007. "Increased myogenic progenitor cell accumulation in CCR2-/- mice despite bone marrow-dependent impairment in muscle regeneration and macrophage recruitment."

Contreras-Shannon, V, McManus LM, Shireman PK. Ischemia-induced enhanced transcription factor (myoD and STAT3) expression in MCP-1 -/- mice despite decreased skeletal muscle regeneration, to be presented at the Society of University Surgeons Meeting, San Diego, CA, 2006 Feb.

Pierce A, McManus LM, Shireman PK, Chaudhuri AR. MPO activity is inversely related to protein structure and function in injured and regenerating muscle, presented at The Role of Protein Misfolding and Aggregation on Aging and Age-Related Disorders, Bandera, TX, 2005 Oct.

Shireman PK, Contreras-Shannon V, McManus LM. Macrophages and muscle regeneration after ischemic injury, presented at the North American Vascular Biology Organization Meeting, Chicago, IL, 2005 Jun. p. 163. (Vascular Medicine; vol. 10, no. 2).

Contreras-Shannon V, McManus LM, Kuziel WA, Shireman PK. Alterations in STAT3 expression during skeletal muscle regeneration in CC chemokine receptor 2 knockout (CCR2 -/-) mice, presented at the FASEB Skeletal Muscle Satellite and Stem Cell Conference, Tucson, AZ, 2005 Jun.

Contreras-Shannon V, McManus LM, Kuziel WA, Shireman PK. Alterations in MyoD expression during skeletal muscle regeneration following cardiotoxin (CT) induced injury in CC chemokine receptor 2 (CCR2 -/-) mice, presented at the Experimental Biology Meeting, San Diego, CA, 2005 Apr. p. A486. (FASEB J; vol. 19, no. 4).

Publications:
Sun D, Martinez CO, Ochoa O, Ruiz-Willhite L, Bonilla JR, Centonze VE, Waite LL, Michalek JE, McManus LM, Shireman PK. Bone marrow-derived cell regulation of skeletal muscle regeneration. FASEB J. 2008 September; [Epub ahead of print]

Ochoa O, Torres FM, Shireman PK. Chemokines and diabetic wound healing. J Vasc Surg, in press, anticipated publication date Dec 2007.

Ochoa O, Sun D, Reyes-Reyna SM, Waite LL, Michalek JE, McManus LM, Shireman PK. Delayed angiogenesis and VEGF production in CCR2 -/- mice during impaired skeletal muscle regeneration. Am J Physiol Reg Int Comp Physiol, 2007 May 23; [Epub ahead of print].

Shireman PK. The chemokine system in arteriogenesis and hind limb ischemia. J Vasc Surg, 45(Supplement A):48A-56A, 2007.

Shireman PK, Contreras-Shannon V, Ochoa O, Karia BP, Michalek JE, McManus LM. MCP-1 deficiency causes altered inflammation with impaired skeletal muscle regeneration. J Leuk Biol, 81:775-85, 2007.

Contreras-Shannon V, Ochoa O, Reyes-Reyna SM, Sun D, Michalek JE, Kuziel WA, McManus LM, Shireman PK. Fat accumulation with altered inflammation and regeneration in skeletal muscle of CCR2 -/- mice following ischemic injury. Am J Physiol Cell Physiol, 292:C953-67, 2007.

Lee S, Shin S, Shireman PK, Vasilaki A, Van Remmen H, Csete M. Glutathione-peroxidase-1 null muscle progenitor cells are globally defective. Free Radic Biol Med. 41:1174-84, 2006.

LaFontaine J, Allen M, Davis C, Harkless LB, Shireman PK. Current concepts in diabetic microvascular dysfunction. J Am Podiatr Med Assoc. 96(3):245-52, 2006.

Shireman PK, Contreras-Shannon VE, Reyes-Reyna SM, Robinson SC, McManus LM. MCP-1 parallels inflammatory and regenerative responses in ischemic muscle. J Surg Res. 134(1):145-57, 2006.

Shireman PK, Quinones MP. Differential necrosis despite similar perfusion in mouse strains after ischemia, J Surg Res, 2005 Dec

Toursarkissian B, Hagino RT, Khan K, Schoolfield J, Shireman PK, Harkless L. Healing of transmetatarsal amputation in the diabetic patient: is angiography predictive? Ann Vasc Surg 2005 Nov:19(6):769-73.

Biosketch: Dr. Shireman obtained her medical degree from Indiana University School of Medicine in Indianapolis, IN in 1990 and completed her Vascular Surgery training at Loyola University Medical Center in Maywood, IL in 1998. She is currently certified with the American Board of Surgery in both General Surgery and Vascular Surgery. Dr. Shireman joined the Vascular Surgery Division at the Health Science Center in 1999. After arriving at the Health Science Center, Dr. Shireman completed her masters program in Clinical Investigation and received numerous grants for research from the NIH, the Department of Veteran Affairs, Howard Hughes Medical Institute, the American Heart Association, and the University of Texas Health Science Center at San Antonio. In 2004 Dr. Shireman was appointed to the faculty for the Barshop Institute for Longevity and Aging Studies.

Awards:
2004, Outstanding Alumni Award, Purdue University, West Lafayette, IN
2004, Presidential Junior Research Scholar Award, University of Texas Health Science Center at San Antonio
2001, Alumni Hidden Diamond Award, Purdue University, West Lafayette, IN
1999, John L. Keeley Surgical Fellowship Award, Loyola University Medical School, Maywood, IL
1998, Gutherie Award for Research, Midwestern Vascular Surgical Society, Dearborn, MI
1997, Research Award, 3rd Place, American Venous Forum, San Antonio, TX
1995, Residents' Research Award, The Chicago Surgical Society, Chicago, IL

Committees, Professional Societies:
Association of Women Surgeons, member
Association for Academic Surgery, member
American College of Surgeons, fellow
The Association of Veterans Administrative Surgeons, member
North American Vascular Biology Organization (NAVBO), member
Society of Vascular Surgery, member
4B Study Section Member, American Heart Association, Western Consortium
Member, Research and Education Committee, American Vascular Association
American Society for Investigative Pathology
Member, Research Allocations & Advisory Committee, American Heart Assoc., TX Affiliate
San Antonio Regenerative Medicine Association, member and collaborative investigator
Society of University Surgeons